Lipopolysaccharide binding protein-deficient mice have a normal defense against pulmonary mycobacterial infection

Judith Branger; Jaklien C Leemans; Sandrine Florquin; Peter Speelman; Douglas T Golenbock; Tom van der Poll

doi:10.1016/j.clim.2005.03.014

Lipopolysaccharide binding protein-deficient mice have a normal defense against pulmonary mycobacterial infection

Clin Immunol. 2005 Aug;116(2):174-81. doi: 10.1016/j.clim.2005.03.014.

Authors

Judith Branger¹, Jaklien C Leemans, Sandrine Florquin, Peter Speelman, Douglas T Golenbock, Tom van der Poll

Affiliation

¹ Department of Experimental Internal Medicine, Academic Medical Center, Room F4-222, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. [email protected]

PMID: 15993364
DOI: 10.1016/j.clim.2005.03.014

Abstract

Lipopolysaccharide (LPS) binding protein (LBP) facilitates the transfer of LPS of Gram-negative bacteria to the pattern recognition receptor CD14, resulting in activation of immunocompetent cells. LBP can also facilitate the binding of lipoarabinomannan, a major cell wall component of mycobacteria, to immune cells. To determine the role of LBP in the immune response to pulmonary Mycobacterium tuberculosis infection, LBP gene-deficient (-/-) and normal wild-type (WT) mice were intranasally infected with M. tuberculosis. LBP-/- mice displayed a similar survival and mycobacterial outgrowth in lungs and liver, although they demonstrated a reduced lymphocyte recruitment and activation during the early stages of infection. The clearance of pulmonary infection with the non-pathogenic M. smegmatis was also unaltered in LBP-/- mice. These data suggest that LBP does not contribute to an effective host response in M. tuberculosis infection.

MeSH terms

Acute-Phase Proteins / deficiency
Acute-Phase Proteins / genetics
Acute-Phase Proteins / immunology*
Animals
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / immunology
Carrier Proteins / genetics
Carrier Proteins / immunology*
Cell Count
Chemokines / metabolism
Cytokines / metabolism
Female
Granulocytes / pathology
Liver / microbiology
Lung / metabolism
Lung / microbiology
Lung / pathology
Lymphocyte Activation / immunology
Lymphocytes / pathology
Macrophages / pathology
Membrane Glycoproteins / deficiency
Membrane Glycoproteins / genetics
Membrane Glycoproteins / immunology*
Mice
Mice, Inbred C57BL
Mice, Knockout
Mycobacterium Infections, Nontuberculous / immunology
Mycobacterium Infections, Nontuberculous / microbiology
Mycobacterium smegmatis / immunology
Survival Analysis
Tuberculosis, Pulmonary / immunology*
Tuberculosis, Pulmonary / microbiology
Tuberculosis, Pulmonary / pathology

Substances

Acute-Phase Proteins
Carrier Proteins
Chemokines
Cytokines
Membrane Glycoproteins
lipopolysaccharide-binding protein