Abstract
The F(1)F(o)-ATP synthase plays an important role in a number of vital cellular processes in plants, animals, and microorganisms. In this study, we constructed a DeltaatpD mutant of Mycobacterium smegmatis and demonstrated that atpD encoding the beta subunit of the F(1)F(o)-ATP synthase is an essential gene in M. smegmatis during growth on nonfermentable and fermentable carbon sources.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Primers
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Gene Deletion
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Mycobacterium smegmatis / enzymology*
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Mycobacterium smegmatis / genetics
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Mycobacterium smegmatis / growth & development
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Plasmids / genetics
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Protein Subunits / genetics
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Proton-Translocating ATPases / genetics*
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Proton-Translocating ATPases / metabolism*
Substances
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DNA Primers
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Protein Subunits
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Proton-Translocating ATPases