Use of a locked-nucleic-acid oligomer in the clamped-probe assay for detection of a minority Pfcrt K76T mutant population of Plasmodium falciparum

J Clin Microbiol. 2005 Jul;43(7):3304-8. doi: 10.1128/JCM.43.7.3304-3308.2005.

Abstract

Given the emergence of drug resistance and the high rate of polyclonal microorganism infections, the availability of a fast and sensitive test to detect minority mutant populations would be an improvement in the diagnosis of infectious diseases. A clamped-probe real-time PCR assay to diagnose the Plasmodium falciparum K76T mutation in clone populations was developed, using a wild-type-specific locked-nucleic-acid-containing oligomer to suppress wild-type PCR amplification and to enhance melting analysis with a mutation-specific detection probe.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Antimalarials / pharmacology
  • Chloroquine / pharmacology
  • Codon
  • DNA, Protozoan / analysis
  • Drug Resistance / genetics*
  • Humans
  • Malaria, Falciparum / parasitology
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Membrane Transport Proteins
  • Mutation*
  • Parasitic Sensitivity Tests / methods
  • Peptide Nucleic Acids*
  • Plasmodium falciparum / drug effects*
  • Plasmodium falciparum / genetics
  • Polymerase Chain Reaction / methods*
  • Protozoan Proteins
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Antimalarials
  • Codon
  • DNA, Protozoan
  • Membrane Proteins
  • Membrane Transport Proteins
  • Peptide Nucleic Acids
  • PfCRT protein, Plasmodium falciparum
  • Protozoan Proteins
  • Chloroquine