Abstract
To improve the production of the lantibiotic subtilin in Bacillus subtilis ATCC 6633, two genetic engineering strategies were followed. Firstly, additional copies of subtilin self-protection (immunity) genes spaIFEG have been integrated into the genome of the producer strain. Their expression significantly enhanced the subtilin tolerance level, and concomitantly, the subtilin yield 1.7-fold. Secondly, a repressor of subtilin gene expression, the B. subtilis general transition state regulator protein AbrB, was deleted. A sixfold enhancement of the subtilin yield could be achieved with the abrB deletion mutant; however, the produced subtilin fraction predominantly consists of succinylated subtilin species with less antimicrobial activity compared to unmodified subtilin.
MeSH terms
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ATP-Binding Cassette Transporters / genetics
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Anti-Bacterial Agents / biosynthesis
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology
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Bacillus subtilis / genetics*
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Bacillus subtilis / metabolism
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Bacterial Proteins / genetics
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Bacteriocins / biosynthesis*
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Bacteriocins / chemistry
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Bacteriocins / genetics
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Bacteriocins / pharmacology
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DNA-Binding Proteins / genetics
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Drug Tolerance
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Gene Deletion
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Gene Dosage
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Genetic Engineering
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Genome, Bacterial
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Lipoproteins / genetics
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Membrane Proteins / genetics
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Peptides / chemistry
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Peptides / genetics
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Peptides / pharmacology
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Succinic Acid / analysis
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Transcription Factors / genetics
Substances
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ATP-Binding Cassette Transporters
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AbrB protein, Bacillus subtilis
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Anti-Bacterial Agents
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Bacterial Proteins
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Bacteriocins
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DNA-Binding Proteins
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Lipoproteins
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Membrane Proteins
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Peptides
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SpaB protein, Bacillus subtilis
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SpaF protein, Bacillus subtilis
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SpaG protein, Bacillus subtilis
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SpaI protein, Bacillus subtilis
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Transcription Factors
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Succinic Acid
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subtilin