Abstract
The temporal control of CONSTANS (CO) expression and activity is a key mechanism in photoperiodic flowering in Arabidopsis. FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1) protein regulates CO transcription, although the molecular mechanism is unknown. We demonstrate here that FKF1 controls the stability of a Dof transcription factor, CYCLING DOF FACTOR 1 (CDF1). FKF1 physically interacts with CDF1, and CDF1 protein is more stable in fkf1 mutants. Plants with elevated levels of CDF1 flower late and have reduced expression of CO. CDF1 and CO are expressed in the same tissues, and CDF1 binds to the CO promoter. Thus, FKF1 controls daily CO expression in part by degrading CDF1, a repressor of CO transcription.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics
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Arabidopsis / growth & development
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Arabidopsis / metabolism*
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Arabidopsis Proteins / biosynthesis
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism*
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Cell Nucleus / metabolism
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics*
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Flowers / growth & development
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Gene Expression Profiling
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Gene Expression Regulation, Plant*
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Molecular Sequence Data
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Mutation
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Phenotype
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Plants, Genetically Modified
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Proteasome Endopeptidase Complex / metabolism
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RNA Interference
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Transcription Factors / biosynthesis
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Transcription Factors / genetics*
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Transcription, Genetic
Substances
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Arabidopsis Proteins
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CDF1 protein, Arabidopsis
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CONSTANS protein, Arabidopsis
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DNA-Binding Proteins
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FKF1 protein, Arabidopsis
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Recombinant Fusion Proteins
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Repressor Proteins
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Transcription Factors
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Proteasome Endopeptidase Complex