Abstract
Bone marrow stromal cells (MSCs) have great potential as therapeutic agents. We report a method for inducing skeletal muscle lineage cells from human and rat general adherent MSCs with an efficiency of 89%. Induced cells differentiated into muscle fibers upon transplantation into degenerated muscles of rats and mdx-nude mice. The induced population contained Pax7-positive cells that contributed to subsequent regeneration of muscle upon repetitive damage without additional transplantation of cells. These MSCs represent a more ready supply of myogenic cells than do the rare myogenic stem cells normally found in muscle and bone marrow.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow Cells / cytology*
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Bone Marrow Cells / physiology
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Bone Marrow Transplantation
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Cell Differentiation*
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Cell Fusion
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Cell Lineage
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Cell Proliferation
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Cell Separation
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Cells, Cultured
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Colforsin / pharmacology
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Fibroblast Growth Factor 2 / pharmacology
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Gene Expression Profiling
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Homeodomain Proteins / analysis
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Humans
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Mice
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Mice, Inbred mdx
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Mice, Nude
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Muscle Cells / cytology*
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Muscle Development / genetics
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Muscle Fibers, Skeletal / cytology*
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Muscle Proteins / analysis
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Muscle, Skeletal / cytology
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Muscular Diseases / therapy*
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Muscular Dystrophy, Duchenne / therapy
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Neuregulins / pharmacology
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PAX7 Transcription Factor
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Platelet-Derived Growth Factor / pharmacology
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Rats
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Regeneration
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Satellite Cells, Skeletal Muscle / cytology
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Satellite Cells, Skeletal Muscle / physiology
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Stem Cells / cytology
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Stem Cells / physiology
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Stromal Cells / cytology*
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Stromal Cells / physiology
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Stromal Cells / transplantation
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Transfection
Substances
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Homeodomain Proteins
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Muscle Proteins
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Neuregulins
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PAX7 Transcription Factor
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PAX7 protein, human
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Pax7 protein, mouse
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Platelet-Derived Growth Factor
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Fibroblast Growth Factor 2
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Colforsin