Abstract
In human cells, the ELL family of transcription factors includes at least three members, which are all capable of stimulating the overall rate of elongation by RNA polymerase II by suppressing transient pausing by the enzyme at many sites along DNA. In this report, we identify the ELL-associated factors (EAF)1 and EAF2 as strong positive regulators of ELL elongation activity. Our findings provide insights into the structure and function of ELL family transcription factors, and they bring to light direct roles for the EAF proteins in regulation of RNA polymerase II transcription.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cells, Cultured
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Cloning, Molecular
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DNA / metabolism*
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DNA-Binding Proteins / metabolism*
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Escherichia coli
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Gene Expression Regulation / physiology*
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Humans
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Insecta
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Neoplasm Proteins / metabolism*
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Peptide Elongation Factors / metabolism*
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Recombinant Proteins / metabolism
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Transcription Factors / metabolism*
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Transcriptional Elongation Factors
Substances
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DNA-Binding Proteins
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EAF1 protein, human
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EAF2 protein, human
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ELL protein, human
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Neoplasm Proteins
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Peptide Elongation Factors
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Recombinant Proteins
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Transcription Factors
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Transcriptional Elongation Factors
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DNA