Abstract
Bone marrow-derived mesenchymal stem cells (bMSCs) can differentiate into a number of different cell/tissue types, and also possess immunoregulatory functions. The present study was undertaken to elucidate the exact immunoregulatory effects of allogeneic bMSCs on T- and B-lymphocyte proliferation, activation, and function maturation of BXSB mice, which has been considered as a experimental model for human systemic lupus erythematosus (SLE). We determined that bMSCs from BALB/c mice had inhibitory effects on BXSB mice T-lymphocyte proliferation, but no inhibitory effect on their activation. In addition, they had a significant inhibitory and stimulatory effect on IL-4- and IFN-gamma-producing T cells, respectively. Also, bMSCs had inhibitory effects on the proliferation, activation, and IgG secretion of B lymphocytes. In addition, BALB/c bMSCs had an enhancing effect on CD40 expression and inhibitory effects on CD40 ligand (CD40L) ectopic hyperexpression on B cells from BXSB mice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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B-Lymphocytes / cytology
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B-Lymphocytes / drug effects
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B-Lymphocytes / immunology*
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Bone Marrow Cells / cytology*
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CD40 Antigens / metabolism
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CD40 Ligand / metabolism
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Cell Proliferation / drug effects
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Cells, Cultured
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Coculture Techniques
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Concanavalin A / pharmacology
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Disease Models, Animal
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Dose-Response Relationship, Drug
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Flow Cytometry
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Genetic Markers / drug effects
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Immunoglobulin G / metabolism
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Interferon-gamma / metabolism
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Interleukin-4 / metabolism
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Lipopolysaccharides / pharmacology
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Lupus Erythematosus, Systemic / immunology
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Lymphocyte Activation / drug effects
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Mesenchymal Stem Cells / immunology*
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Mice
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Mice, Inbred BALB C
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Mice, Mutant Strains
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Mitogens / pharmacology
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Spleen / cytology
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T-Lymphocytes / cytology
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T-Lymphocytes / drug effects
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T-Lymphocytes / immunology*
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Transplantation, Homologous / immunology*
Substances
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CD40 Antigens
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Genetic Markers
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Immunoglobulin G
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Lipopolysaccharides
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Mitogens
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Concanavalin A
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CD40 Ligand
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Interleukin-4
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Interferon-gamma