The efficiency of sperm washing in removing human immunodeficiency virus type 1 varies according to the seminal viral load

Josè Ramòn Fiore; Filomenamila Lorusso; Margherita Vacca; Nicoletta Ladisa; Pantaleo Greco; Raffaella De Palo

doi:10.1016/j.fertnstert.2004.12.060

The efficiency of sperm washing in removing human immunodeficiency virus type 1 varies according to the seminal viral load

Fertil Steril. 2005 Jul;84(1):232-4. doi: 10.1016/j.fertnstert.2004.12.060.

Authors

Josè Ramòn Fiore¹, Filomenamila Lorusso, Margherita Vacca, Nicoletta Ladisa, Pantaleo Greco, Raffaella De Palo

Affiliation

¹ Clinic of Infectious Diseases University of Bari, Bari, Italy. [email protected]

PMID: 16009191
DOI: 10.1016/j.fertnstert.2004.12.060

Abstract

We evaluated the relationship between the seminal HIV-1 viral load and the efficiency of a standardized sperm-washing procedure in removing HIV-1 RNA from semen samples. The results obtained indicate that the amount of virus present in the original sample affects the efficiency of the procedure and suggest that the seminal viral load should be preevaluated before enrolling an HIV-serodiscordant couple in an assisted reproduction protocol.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Fertilization in Vitro / methods
HIV Infections / virology
HIV-1*
Humans
Male
Semen / virology*
Specimen Handling / methods
Spermatozoa / virology*
Viral Load* / statistics & numerical data