Facilitating genome navigation: survey sequencing and dense radiation-hybrid gene mapping

Nat Rev Genet. 2005 Aug;6(8):643-8. doi: 10.1038/nrg1658.

Abstract

Accurate and comprehensive sequence coverage for large genomes has been restricted to only a few species of specific interest. Lower sequence coverage (survey sequencing) of related species can yield a wealth of information about gene content and putative regulatory elements. But survey sequences lack long-range continuity and provide only a fragmented view of a genome. Here we show the usefulness of combining survey sequencing with dense radiation-hybrid (RH) maps for extracting maximum comparative genome information from model organisms. Based on results from the canine system, we propose that from now on all low-pass sequencing projects should be accompanied by a dense, gene-based RH map-construction effort to extract maximum information from the genome with a marginal extra cost.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Dogs
  • Humans
  • Phylogeny
  • Radiation Hybrid Mapping*
  • Sequence Analysis, DNA*