Abstract
Under iron deprivation Omphalotus olearius was found to produce the hydroxamate siderophore ferrichrome A. A gene cluster consisting of three genes: fso1, a nonribosomal peptide synthetase whose expression is enhanced in the absence of iron; omo1, a l-ornithine-N(5)-monooxygenase; and ato1, an acyltransferase probably involved in the transfer of the methylglutaconyl residue to N(5)-hydroxyorinithine was identified. The fso1 sequence is interrupted by 48 introns and its derived protein sequence has a similar structure to the homologous genes of Ustilago maydis and Aspergillus nidulans. This is the first report of a nonribosomal peptide synthetase gene and a biosynthetic gene cluster in homobasidiomycetes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acyltransferases / genetics
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Acyltransferases / metabolism
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Agaricales / genetics
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Agaricales / growth & development
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Agaricales / metabolism*
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Amino Acid Sequence
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Ferrichrome / metabolism*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Gene Expression Regulation, Fungal
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Iron / metabolism
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Mixed Function Oxygenases / genetics
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Mixed Function Oxygenases / metabolism
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Molecular Sequence Data
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Multigene Family*
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Peptide Synthases / genetics
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Peptide Synthases / metabolism
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Sequence Analysis, DNA
Substances
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Fungal Proteins
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Ferrichrome
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Iron
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Mixed Function Oxygenases
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ornithine N5-oxygenase
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Acyltransferases
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Peptide Synthases
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non-ribosomal peptide synthase
Associated data
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GENBANK/AY929616
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GENBANK/AY929617
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GENBANK/AY929618