Abstract
Using a cell fusion assay, we show here that in addition to complete fusion SNAREs also promote hemifusion as an alternative outcome. Approximately 65% of events resulted in full fusion, and the remaining 35% in hemifusion; of those, approximately two thirds were permanent and approximately one third were reversible. We predict that this relatively close balance among outcomes could be tipped by binding of regulatory proteins to the SNAREs, allowing for dynamic physiological regulation between full fusion and reversible kiss-and-run-like events.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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CHO Cells
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Cell Membrane / physiology
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Cricetinae
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Cricetulus
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Membrane Fusion / genetics
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Membrane Fusion / physiology*
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Mice
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Protein Conformation
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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SNARE Proteins
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Vesicular Transport Proteins / chemistry
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Vesicular Transport Proteins / genetics
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Vesicular Transport Proteins / metabolism*
Substances
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Recombinant Proteins
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SNARE Proteins
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Vesicular Transport Proteins