Abstract
The lineage of dendritic cells (DC), and in particular their relationship to monocytes and macrophages, remains obscure. Furthermore, the requirement for the macrophage growth factor CSF-1 during DC homeostasis is unclear. Using a transgenic mouse in which the promoter for the CSF-1R (c-fms) directs the expression of enhanced GFP in cells of the myeloid lineage, we determined that although the c-fms promoter is inactive in DC precursors, it is up-regulated in all DC subsets during differentiation. Furthermore, plasmacytoid DC and all CD11c(high) DC subsets are reduced by 50-70% in CSF-1-deficient osteopetrotic mice, confirming that CSF-1 signaling is required for the optimal differentiation of DC in vivo. These data provide additional evidence that the majority of tissue DC is of myeloid origin during steady state and supports a close relationship between DC and macrophage biology in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blood Cell Count
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CD11c Antigen / biosynthesis
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CD11c Antigen / blood
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Cell Differentiation / genetics
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Cell Differentiation / immunology*
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Cell Proliferation*
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Cells, Cultured
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Dendritic Cells / cytology
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Hematopoietic Stem Cells / cytology
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Hematopoietic Stem Cells / immunology
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Hematopoietic Stem Cells / metabolism
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Leukocyte Common Antigens / biosynthesis
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Macrophage Colony-Stimulating Factor / deficiency
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Macrophage Colony-Stimulating Factor / genetics
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Macrophage Colony-Stimulating Factor / metabolism*
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Mice
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Mice, Knockout
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Mice, Transgenic
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Receptor, Macrophage Colony-Stimulating Factor / biosynthesis*
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Receptor, Macrophage Colony-Stimulating Factor / physiology
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Signal Transduction / genetics
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Signal Transduction / immunology
Substances
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CD11c Antigen
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Macrophage Colony-Stimulating Factor
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Receptor, Macrophage Colony-Stimulating Factor
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Leukocyte Common Antigens