Characterization of monoclonal antibodies against human leukocyte 5-lipoxygenase

Prostaglandins Leukot Essent Fatty Acids. 1992 Apr;45(4):259-65. doi: 10.1016/0952-3278(92)90081-s.

Abstract

Four mouse monoclonal IgG1 antibody-producing cell lines (5LO-1, 5LO-2, 5LO-3, 5LO-4), produced against highly purified human leukocyte 5-lipoxygenase have been characterized. The monoclonal antibodies produced by these cell lines exhibited differential reactivity against 5-lipoxygenase as determined by ELISA and immunoprecipitation analyses. Monoclonal antibodies 5LO-2 and 5LO-3 inhibited the activity of recombinant human leukocyte 5-lipoxygenase in a dose-dependent manner. This inhibition was selective for 5-lipoxygenase activity since these monoclonal antibodies did not inhibit human leukocyte 15-lipoxygenase or porcine leukocyte 12-lipoxygenase.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antibody Specificity
  • Arachidonate 5-Lipoxygenase / immunology*
  • Humans
  • Hybridomas / immunology
  • Leukocytes / enzymology
  • Lipoxygenase Inhibitors
  • Mice
  • Mice, Inbred BALB C
  • Rats
  • Rats, Inbred Strains

Substances

  • Antibodies, Monoclonal
  • Lipoxygenase Inhibitors
  • Arachidonate 5-Lipoxygenase