Abstract
A new class of bacterial multisubunit membrane-bound electron-transfer complexes has been identified based on biochemical and bioinformatic data. It contains subunits homologous to the three-subunit molybdopterin oxidoreductases and four additional subunits, two of which are c-type cytochromes. The complex was purified from the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus, and putative operons for similar complexes were identified in a wide range of bacteria. In most cases, the presence of the new complex is anticorrelated with the cytochrome bc or bf electron-transfer complex, suggesting that it replaces it functionally. This appears to be a widespread yet previously unrecognized protein complex involved in energy metabolism in bacteria.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry*
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Bacterial Proteins / classification*
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Bacterial Proteins / genetics
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Chloroflexus / enzymology*
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Chloroflexus / genetics
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Computational Biology
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Cytochromes c / chemistry
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Cytochromes c / classification
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Cytochromes c / genetics
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Electron Transport / genetics
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Electron Transport Complex IV / chemistry*
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Electron Transport Complex IV / classification*
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Electron Transport Complex IV / genetics
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Genome, Bacterial
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Membrane Proteins / chemistry*
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Membrane Proteins / classification*
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Membrane Proteins / genetics
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Molecular Sequence Data
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Multigene Family
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Operon
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Phylogeny
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Protein Subunits / chemistry
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Protein Subunits / classification
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Protein Subunits / genetics
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Structural Homology, Protein
Substances
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Bacterial Proteins
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Membrane Proteins
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Protein Subunits
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Cytochromes c
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Electron Transport Complex IV