Allosterically activated Diels-Alder catalysis by a ribozyme

Mark Helm; Markus Petermeier; Bixia Ge; Roberto Fiammengo; Andres Jäschke

doi:10.1021/ja052886i

Allosterically activated Diels-Alder catalysis by a ribozyme

J Am Chem Soc. 2005 Aug 3;127(30):10492-3. doi: 10.1021/ja052886i.

Authors

Mark Helm¹, Markus Petermeier, Bixia Ge, Roberto Fiammengo, Andres Jäschke

Affiliation

¹ Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.

PMID: 16045328
DOI: 10.1021/ja052886i

Abstract

We describe the allosteric control of Diels-Alder reactions by a small organic effector, theophylline. This is achieved by converting a Diels-Alder ribozyme into an allosterically regulated system. In contrast to other published systems, we have a bond-forming reaction with two small-molecule substrates and multiple turnover. This system could be very attractive for the development of assays for a variety of analytes and can be regarded as a prototype of fully synthetic signaling cascades.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthracenes / chemistry*
Base Sequence
Biotin / chemistry
Catalysis
DNA-Directed RNA Polymerases / chemistry
Kinetics
Maleimides / chemistry*
Molecular Sequence Data
Nucleic Acid Conformation
Nucleotides / chemistry
RNA / chemistry*
RNA, Catalytic / chemistry*
RNA, Catalytic / metabolism
Theophylline / chemistry
Viral Proteins / chemistry

Substances

Anthracenes
Maleimides
Nucleotides
RNA, Catalytic
Viral Proteins
RNA
Biotin
Theophylline
bacteriophage T7 RNA polymerase
DNA-Directed RNA Polymerases
anthracene