LT, VP1 and TCR-BKV sequence analysis in a patient with post-transplant BKV nephropathy associated with EBV-related PTLD

Pediatr Nephrol. 2005 Oct;20(10):1506-9. doi: 10.1007/s00467-005-1963-0. Epub 2005 Jul 27.

Abstract

A 10-year-old boy kidney transplant recipient (KTR) developed an abdominal post-transplant lymphoproliferative syndrome (PTLD) followed by BK virus nephropathy (BKVN). BK virus (BKV) and Epstein-Barr virus (EBV) were studied in renal and PTLD tissue by polymerase chain reaction (PCR) assay. Afterwards, the patient was monitored in relation to BKV in urine and serum; transcription control region (TCR)-BK polymorphism sequence analysis was also performed. In the PCR assay, both early large T antigen (LT) and late (VP1) transcriptional BKV coding regions were found in renal tissue, whereas EBV and only LT-BK were detected in PTLD abdominal tissue. On the other hand, TCR sequence analysis revealed the AS genomic BK variant.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Viral, Tumor / genetics
  • BK Virus / genetics*
  • Child
  • Epstein-Barr Virus Infections / complications*
  • Humans
  • Kidney Diseases / virology*
  • Kidney Transplantation / adverse effects*
  • Lymphoproliferative Disorders / etiology*
  • Male
  • Polyomavirus Infections / complications*
  • Regulatory Sequences, Nucleic Acid / genetics
  • Sequence Analysis
  • Transcription, Genetic / genetics
  • Tumor Virus Infections / complications*

Substances

  • Antigens, Viral, Tumor