Abstract
In reporter-based transient expression systems, we characterized simian immunodeficiency virus from a chimpanzee (SIVCPZ), with special reference to the human immunodeficiency virus type 1 (HIV-1). SIVCPZ was not equally activated by tat and rev transactivators derived from representative primate lentiviruses. HIV-1 alone activated SIVCPZ to the full extent in both tat and rev assays. The tat and rev gene products of SIVCPZ, as well as those of HIV-1, efficiently transactivated the other viruses. These results indicate that SIVCPZ is identical to HIV-1 with regard to the compatibility of tat and rev gene activities among four subgroups of primate lentiviruses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chloramphenicol O-Acetyltransferase / biosynthesis
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DNA, Recombinant / genetics
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Gene Expression Regulation, Viral*
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Gene Products, rev / biosynthesis
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Gene Products, rev / genetics
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Gene Products, tat / biosynthesis
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Gene Products, tat / genetics
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HIV-1 / classification
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HIV-1 / genetics
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Molecular Sequence Data
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Nucleic Acid Conformation
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Pan troglodytes
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RNA, Viral / chemistry
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Sequence Homology, Nucleic Acid
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Simian Immunodeficiency Virus / classification
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Simian Immunodeficiency Virus / genetics*
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Simian Immunodeficiency Virus / isolation & purification
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Trans-Activators / biosynthesis
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Trans-Activators / genetics*
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rev Gene Products, Human Immunodeficiency Virus
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tat Gene Products, Human Immunodeficiency Virus
Substances
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DNA, Recombinant
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Gene Products, rev
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Gene Products, tat
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RNA, Viral
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Trans-Activators
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rev Gene Products, Human Immunodeficiency Virus
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tat Gene Products, Human Immunodeficiency Virus
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Chloramphenicol O-Acetyltransferase
Associated data
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GENBANK/M19921
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GENBANK/M30895
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GENBANK/M52154
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GENBANK/X07805
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GENBANK/X15781