Two F2 populations with different kinds of spike derived from maize male sterility materials RP(3)195 (A) x S37 (inbred line) ,which had been sib-bred for many generations,were used for sterility analysis and gene location. There were 138 and 247 plants in the two F2 populations respectively. Among the 326 pairs of microsatellite primers selected,56 were found polymorphic. Linkage analysis of F2 populations with the 56 pairs of primers showed that microsatellite markers bnlg197 and umc1012 were linked with the male sterility gene. The genetic distance between marker bnlgl97 and the male sterility gene in the two different F2 populations were 7 cM and 14.5 cM respectively. The genetic distance between marker umc1012 and the male sterility gene in the 138 plants was 28.5 cM. Thus the male sterility gene was located on chromosome 3L.