Abstract
Cationic liposomes formulated with neutral 1,2-dimyristoyl-sn-glycero-3-phosphocholine and cationic gemini surfactants were used for transfecting different cell lines with a reporter gene. The efficiency in the transfection has been correlated to the high extent of DNA condensation observed by circular dichroism, condensation shown to depend heavily on the gemini spacer structure. Transfection efficiency was better than that obtained with a commercial lipofection kit.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Butylamines / chemistry
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Cations
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Cell Line
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Chlorocebus aethiops
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Circular Dichroism
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Cytomegalovirus / genetics
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DNA / administration & dosage*
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DNA / chemistry
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Dimyristoylphosphatidylcholine / chemistry*
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Ethylamines / chemistry
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Genes, Reporter
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Green Fluorescent Proteins / genetics
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Humans
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Liposomes / chemistry*
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Promoter Regions, Genetic
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Quaternary Ammonium Compounds / chemistry*
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Rats
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Structure-Activity Relationship
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Surface-Active Agents / chemistry*
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Transfection*
Substances
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Butylamines
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Cations
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Ethylamines
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Liposomes
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Quaternary Ammonium Compounds
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Surface-Active Agents
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Green Fluorescent Proteins
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DNA
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Dimyristoylphosphatidylcholine