Approaches to bacterial RNA isolation and purification for microarray analysis of Escherichia coli K1 interaction with human brain microvascular endothelial cells

Francescopaolo Di Cello; Yi Xie; Maneesh Paul-Satyaseela; Kwang Sik Kim

doi:10.1128/JCM.43.8.4197-4199.2005

Approaches to bacterial RNA isolation and purification for microarray analysis of Escherichia coli K1 interaction with human brain microvascular endothelial cells

J Clin Microbiol. 2005 Aug;43(8):4197-9. doi: 10.1128/JCM.43.8.4197-4199.2005.

Authors

Francescopaolo Di Cello¹, Yi Xie, Maneesh Paul-Satyaseela, Kwang Sik Kim

Affiliation

¹ Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Park 256, Baltimore, MD 21287, USA.

Abstract

We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in the gene expression pattern during the RNA isolation procedure.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Brain / blood supply
Brain / microbiology*
Endothelial Cells / microbiology*
Escherichia coli / genetics*
Gene Expression Profiling
Humans
Microcirculation / microbiology
Oligonucleotide Array Sequence Analysis / methods*
RNA, Bacterial / isolation & purification*

Substances

RNA, Bacterial

Abstract

Publication types

MeSH terms

Substances

Grants and funding