Affordable, abbreviated roche monitor assay for quantification of human immunodeficiency virus type 1 RNA in plasma

J Clin Microbiol. 2005 Aug;43(8):4200-2. doi: 10.1128/JCM.43.8.4200-4202.2005.

Abstract

The cost for the Roche Monitor assay kit can be reduced 50% by using only the 1:1, 1:25, and 1:625 human immunodeficiency virus (HIV) and the 1:1 quantitation standard dilutions. This abbreviated test applied to 1,774 mostly African samples had results nearly identical to those obtained following the package insert instructions. To make this approach feasible, Roche would have to provide additional lysis buffer and master mix.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Female
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Humans
  • Pregnancy
  • Prospective Studies
  • RNA, Viral / blood*
  • Reagent Kits, Diagnostic / economics*
  • Viral Load

Substances

  • RNA, Viral
  • Reagent Kits, Diagnostic