Generation of embryoid bodies from mouse embryonic stem cells cultured on STO feeder cells

Qing-Jun Zhou; Jian-Zhong Shao; Li-Xin Xiang; Ruo-Zhen Hu; Yong-Liang Lu; Hang Yao; Li-Cheng Dai

doi:10.1016/j.cellbi.2005.05.007

Generation of embryoid bodies from mouse embryonic stem cells cultured on STO feeder cells

Cell Biol Int. 2005 Sep;29(9):817-25. doi: 10.1016/j.cellbi.2005.05.007.

Authors

Qing-Jun Zhou¹, Jian-Zhong Shao, Li-Xin Xiang, Ruo-Zhen Hu, Yong-Liang Lu, Hang Yao, Li-Cheng Dai

Affiliation

¹ College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310012, PR China.

PMID: 16085433
DOI: 10.1016/j.cellbi.2005.05.007

Abstract

Embryoid bodies, which are similar to post-implantation egg-cylinder stage embryos, provide a model for the study of embryo development and stem cell differentiation. We describe here a novel method for generating embryoid bodies from murine embryonic stem (ES) cells cultured on the STO feeder layer. The ES cells grew into compact aggregates in the first 3 days of coculture, then became simple embryoid bodies (EBs) possessing primitive endoderm on the outer layer. They finally turned into cystic embryoid bodies after being transferred to Petri dishes for 1-3 days. Evaluation of the EBs in terms of morphology and differentiating potential indicates that they were typical in structure and could generate cells derived from the three germ layers. The results show that embryoid bodies can form not only in suspension culture but also directly from ES cells cultured on the STO feeder layer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Butyrates / pharmacology
Cell Aggregation
Cell Differentiation* / drug effects
Cell Line
Cell Lineage
Coculture Techniques / methods*
Dimethyl Sulfoxide / pharmacology
Embryo, Mammalian / cytology*
Embryo, Mammalian / drug effects
Embryo, Mammalian / ultrastructure
GATA4 Transcription Factor / analysis
GATA4 Transcription Factor / genetics
Germ Layers / cytology
Germ Layers / drug effects
Germ Layers / ultrastructure
Hepatocytes / chemistry
Hepatocytes / cytology
Mice
Microscopy, Electron, Transmission
Myocytes, Cardiac / chemistry
Myocytes, Cardiac / cytology
Neurofilament Proteins / analysis
Neurofilament Proteins / genetics
Neurons / chemistry
Neurons / cytology
RNA, Messenger / analysis
Stem Cells / cytology*
Stem Cells / drug effects
Stem Cells / ultrastructure
Tretinoin / pharmacology
alpha-Fetoproteins / analysis
alpha-Fetoproteins / genetics

Substances

Butyrates
GATA4 Transcription Factor
Gata4 protein, mouse
Neurofilament Proteins
RNA, Messenger
alpha-Fetoproteins
Tretinoin
Dimethyl Sulfoxide