Abstract
A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome c production.
Publication types
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Evaluation Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Cytochromes c / biosynthesis*
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DNA Transposable Elements / genetics*
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Deoxyribonuclease HindIII / metabolism*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Mutagenesis, Insertional*
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Mutation
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Sequence Analysis, DNA
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Shewanella / genetics*
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Shewanella / growth & development
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Shewanella / isolation & purification
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Shewanella / metabolism
Substances
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Bacterial Proteins
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CcmC protein, bacteria
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DNA Transposable Elements
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Membrane Proteins
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Cytochromes c
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Deoxyribonuclease HindIII