Abstract
B cells play an indispensable, yet indeterminate, role in the pathogenesis of multiple sclerosis (MS). We measured mRNA of APRIL-a promotor of B-cell survival-in peripheral blood and quantified protein levels in plasma and cerebrospinal fluid in MS patients and controls. APRIL mRNA levels in monocytes and T cells were significantly higher in MS patients than in controls. Levels of soluble APRIL in plasma were higher in patients with chronic progressive MS than in patients with relapsing-remitting MS, albeit not significantly. MS may thus be associated with increased transcription in peripheral blood of factors promoting B-cell survival, including APRIL.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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B-Lymphocytes / metabolism
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Blotting, Western / methods
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Cell Count / methods
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Child
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Child, Preschool
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Female
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Gene Expression Regulation / physiology*
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Humans
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Infant
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Infant, Newborn
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Leukocytes / metabolism
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Male
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Membrane Proteins / blood*
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Membrane Proteins / cerebrospinal fluid*
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Membrane Proteins / genetics
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Middle Aged
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Multiple Sclerosis / classification
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Multiple Sclerosis / genetics
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Multiple Sclerosis / metabolism*
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Tumor Necrosis Factor Ligand Superfamily Member 13
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Tumor Necrosis Factor-alpha / cerebrospinal fluid*
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Tumor Necrosis Factor-alpha / genetics
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X-Linked Inhibitor of Apoptosis Protein / genetics
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X-Linked Inhibitor of Apoptosis Protein / metabolism
Substances
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Membrane Proteins
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RNA, Messenger
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TNFSF13 protein, human
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Tumor Necrosis Factor Ligand Superfamily Member 13
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Tumor Necrosis Factor-alpha
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X-Linked Inhibitor of Apoptosis Protein
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XIAP protein, human