Abstract
Objectives:
Platelet release of alpha granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated.
Methods:
Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method.
Results:
Beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin.
Conclusions:
Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine / pharmacology
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Adult
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Blood Platelets / drug effects
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Blood Platelets / metabolism*
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Chemokines, CXC / blood
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Chemokines, CXC / metabolism*
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Citric Acid / pharmacology
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Dipyridamole / pharmacology
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Edetic Acid / pharmacology
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Humans
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Platelet Activation / drug effects
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Platelet Aggregation Inhibitors / pharmacology
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Platelet Factor 4 / metabolism*
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Serotonin / blood
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Serotonin / metabolism*
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Theophylline / pharmacology
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beta-Thromboglobulin / metabolism*
Substances
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Chemokines, CXC
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Platelet Aggregation Inhibitors
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beta-Thromboglobulin
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Citric Acid
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Serotonin
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Platelet Factor 4
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Dipyridamole
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Edetic Acid
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Theophylline
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Adenosine