Abstract
Disruption of ppsA, a key gene in gluconeogenesis, of Xanthomonas campestris pv. campestris resulted in the failure of the pathogen to grow in medium with pyruvate or C4-dicarboxylates as the sole carbon source and a significant reduction in virulence, indicating that X. campestris pv. campestris possesses only the malic enzyme-PpsA route in gluconeogenesis, which is required for virulence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Primers
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Dicarboxylic Acids / metabolism
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Gluconeogenesis / genetics*
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Plant Diseases / microbiology
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Plant Leaves / microbiology
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Pyruvates / metabolism
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Sequence Deletion
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Virulence
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Xanthomonas campestris / genetics
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Xanthomonas campestris / growth & development
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Xanthomonas campestris / metabolism*
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Xanthomonas campestris / pathogenicity*
Substances
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DNA Primers
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Dicarboxylic Acids
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Pyruvates
Associated data
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GENBANK/AY618213
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GENBANK/AY724680
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GENBANK/AY724681