Gold nanoparticle-based competitive colorimetric assay for detection of protein-protein interactions

Chem Commun (Camb). 2005 Sep 14:(34):4273-5. doi: 10.1039/b507237a. Epub 2005 Jul 28.

Abstract

A gold nanoparticle-based competitive colorimetric assay uses the ensemble of Concanavalin (ConA) and mannopyranoside-encapsulated gold nanoparticles (Man-GNPs) to identify the binding partners for ConA and the binding constants are determined based on the wavelength shifts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Colorimetry
  • Concanavalin A / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Gold / chemistry*
  • Lectins
  • Nanoparticles*
  • Proteins / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Spectrophotometry, Ultraviolet
  • Surface Plasmon Resonance
  • Thyroglobulin / chemistry

Substances

  • Lectins
  • Proteins
  • Concanavalin A
  • Gold
  • Thyroglobulin