Transplantation of embryonic serotonin immunoreactive neurons into the transected spinal cord of adult monkey (Macaca fascicularis)

Brain Res. 1992 Feb 14;572(1-2):329-34. doi: 10.1016/0006-8993(92)90495-u.

Abstract

Five adult monkeys (Macaca fascicularis) underwent a total section of the spinal cord at the thoracic level (T6). Four of them received a daily treatment with cyclosporin (10 mg/kg). Ten days later, two animals treated with cyclosporin and one without cyclosporin received at T8 and T10 levels an injection of a cell suspension prepared from the rhombencephalon of a 40-day-old macaque embryo. Two control animals received one injection of Hank's balanced salt solution. The animals were sacrificed after 2 months (one grafted and one control) and 3 months (two grafted and one control), and their spinal cord was processed for the immunocytochemical detection of serotonin using light and electron microscopy. After 2 months of survival, serotonergic neurons had survived and developed within the transplant. Three months after transplantation, in the animal treated with cyclosporin, serotonergic neurons were found to survive with their axons growing into the host grey matter and establishing axosomatic and axodendritic synapses in the ventral horn. If the graft was isolated in the white matter no fibers were seen invading the grey matter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Tissue Transplantation / physiology*
  • Cyclosporins / therapeutic use
  • Fetal Tissue Transplantation / physiology*
  • Graft Survival / drug effects
  • Macaca fascicularis
  • Neurons / transplantation*
  • Rhombencephalon / cytology
  • Rhombencephalon / embryology
  • Rhombencephalon / transplantation*
  • Serotonin / physiology
  • Spinal Cord / physiology*

Substances

  • Cyclosporins
  • Serotonin