Generation of a yeast two-hybrid strain suitable for competitive protein binding analysis

Biotechniques. 2005 Aug;39(2):165-6, 168. doi: 10.2144/05392BM01.

Authors

Markus Ralser¹, Heike Goehler, Erich E Wanker, Hans Lehrach, Sylvia Krobitsch

Affiliation

¹ Max Planck Institute for Molecular Genetics, Berlin, Germany.

PMID: 16116786
DOI: 10.2144/05392BM01

No abstract available

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Humans
Huntingtin Protein
Nerve Tissue Proteins / analysis
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Nuclear Proteins / analysis
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Protein Interaction Mapping / methods*
Saccharomyces cerevisiae / classification
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism*
Species Specificity
Two-Hybrid System Techniques*

Substances

HTT protein, human
Huntingtin Protein
Nerve Tissue Proteins
Nuclear Proteins