Abstract
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Biomarkers, Tumor / metabolism*
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Gene Expression Profiling / methods*
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Humans
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In Situ Hybridization, Fluorescence / methods*
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Male
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Neoplasm Proteins / metabolism*
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Paraffin Embedding / methods*
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Prostatic Neoplasms / metabolism*
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Prostatic Neoplasms / pathology*
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Tissue Distribution
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Tumor Cells, Cultured
Substances
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Biomarkers, Tumor
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Neoplasm Proteins