Monitoring the cell cycle by multi-kinase-dependent regulation of Swe1/Wee1 in budding yeast

Cell Cycle. 2005 Oct;4(10):1346-9. doi: 10.4161/cc.4.10.2049. Epub 2005 Oct 22.

Abstract

In eukaryotes, G(2)/M transition is induced by the activation of cyclin B-bound Cdk1, which is held in check by the protein kinase, Wee1. Recent advances in our understanding of mitotic entry in budding yeast has revealed that these cells utilize the level of Swe1 (Wee1 ortholog) phosphorylation as a means of monitoring cell cycle progression and of coordinating morphogenetic events with mitotic entry. Swe1 is phosphorylated by at least three distinct kinases at different stages of the cell cycle. This cumulative phosphorylation leads to the hyperphosphorylation and degradation of Swe1 through ubiquitin-mediated proteolysis. Thus, Swe1 functions as an important cell cycle modulator that integrates multiple upstream signals from prior cell cycle events before its ultimate degradation permits passage into mitosis.

Publication types

  • Review

MeSH terms

  • Cell Cycle Proteins / metabolism*
  • Cell Cycle*
  • Gene Expression Regulation, Fungal
  • Humans
  • Phosphorylation
  • Phosphotransferases / metabolism*
  • Saccharomycetales / cytology*
  • Saccharomycetales / enzymology
  • Saccharomycetales / metabolism*

Substances

  • Cell Cycle Proteins
  • Phosphotransferases