Abstract
A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Infective Agents / pharmacology*
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Blotting, Southern
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Cloning, Molecular
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Cross Infection / microbiology*
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Culture Media
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Drug Resistance, Bacterial
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Escherichia coli / drug effects
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Escherichia coli / genetics
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Folic Acid Antagonists / pharmacology*
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Humans
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Methicillin Resistance
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Molecular Sequence Data
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Reverse Transcriptase Polymerase Chain Reaction
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Staphylococcal Infections / microbiology*
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / genetics
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Tetrahydrofolate Dehydrogenase / genetics*
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Tetrahydrofolate Dehydrogenase / metabolism*
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Trimethoprim / pharmacology*
Substances
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Anti-Infective Agents
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Culture Media
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Folic Acid Antagonists
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Trimethoprim
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Tetrahydrofolate Dehydrogenase