Abstract
Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as functionality of a bilayer membrane. Here, we describe the synthesis of lipids for a more modular design and the adaptation of the linker part to silane chemistry. We were able to form a functional tethered bilayer lipid membrane with good electrical sealing properties covering a silicon oxide surface. We demonstrate the functional incorporation of the ion carrier valinomycin and of the ion channel gramicidin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biophysics / methods*
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Electric Impedance
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Electrochemistry
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Gold / chemistry
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Gramicidin / pharmacology
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Ion Channels / chemistry*
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Ionophores / pharmacology
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Ions
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Lipid Bilayers / chemistry*
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Lipid Bilayers / metabolism
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Lipids / chemistry*
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Microscopy, Atomic Force
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Models, Chemical
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Phosphatidylcholines / chemistry
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Phytol / chemistry
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Protein Array Analysis / methods*
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Silanes / chemistry*
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Silanes / metabolism
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Silicon / chemistry
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Silicon Dioxide / chemistry*
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Spectrophotometry
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Substrate Specificity
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Temperature
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Time Factors
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Valinomycin / chemistry
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Valinomycin / pharmacology
Substances
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2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-chloro-dimethylpropyl-silane) ether lipid
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2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-trichloropropyl-silane) ether lipid
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Ion Channels
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Ionophores
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Ions
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Lipid Bilayers
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Lipids
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Phosphatidylcholines
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Silanes
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Gramicidin
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Phytol
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Valinomycin
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1,2-diphytanoylphosphatidylcholine
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Gold
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Silicon Dioxide
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Silicon