Abstract
Replication competent chimeric viruses that express the gag and pol proteins of SIVmac and the env proteins of HIV-1 were made. One such chimeric virus, SHIV-4, that expresses the vif, vpx, vpr, and nef regulatory genes of SIV and the tat and rev regulatory genes of HIV-1 replicated efficiently in cynomolgus monkeys. This model system can be used to evaluate the efficacy of anti-HIV-1 vaccines directed at the envelope glycoproteins, anti-HIV-1 envelope glycoprotein antiserum or monoclonal antibodies, and anti-HIV-1 drugs designed to inhibit the tat, rev, or env functions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cells, Cultured
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Chimera*
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Female
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Gene Products, env / metabolism*
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HIV Infections / metabolism
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HIV Infections / microbiology*
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HIV-1 / genetics*
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HIV-1 / physiology
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Macaca fascicularis
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Male
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Molecular Sequence Data
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Recombination, Genetic
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Simian Acquired Immunodeficiency Syndrome / metabolism
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Simian Acquired Immunodeficiency Syndrome / microbiology*
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Simian Immunodeficiency Virus / genetics*
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Simian Immunodeficiency Virus / physiology
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Virus Replication