Identification of novel genes in the memory response to Brucella infection by cDNA arrays

Chrysanthi M Paranavitana; Elzbieta Zelazowska; Rina Das; Mina Izadjoo; Marti Jett; David Hoover

doi:10.1016/j.mcp.2005.06.009

Identification of novel genes in the memory response to Brucella infection by cDNA arrays

Mol Cell Probes. 2005 Oct;19(5):341-8. doi: 10.1016/j.mcp.2005.06.009.

Authors

Chrysanthi M Paranavitana¹, Elzbieta Zelazowska, Rina Das, Mina Izadjoo, Marti Jett, David Hoover

Affiliation

¹ Department of Bacterial Diseases, Walter Reed Army Institute of Research, Building 503, Forest Glen, Silver Spring, MD 20910, USA. [email protected]

PMID: 16146685
DOI: 10.1016/j.mcp.2005.06.009

Abstract

This study investigated memory responses in immune mice spleen cells to brucellosis by gene expression utilizing cDNA micro arrays. Out of a total of 1176 cDNA's 21 genes were differentially regulated in three independent experiments, and generally supported a Th1 type immune response. 10 genes were validated by real time PCR, and 3 genes (CD 86, CD 40 L and CD 132) were also analyzed by Flow Cytometry for surface protein expression. We extended these findings by studying the expression of five selected genes (IRF 1, SOCS 1, IL 2 R, IRF 7, and CXCR 4) in two independent groups of Brucella immunized mice. In this study we show the potential application of utilizing gene arrays to identify and establish new correlates of protection against a cell mediated immune response.

MeSH terms

Animals
Brucella / immunology*
Brucellosis / immunology*
Carrier Proteins / genetics
Carrier Proteins / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Gene Expression Profiling
Gene Expression Regulation*
Humans
Immunologic Memory / genetics*
Interferon Regulatory Factor-1
Interferon Regulatory Factor-7
Interleukin Receptor Common gamma Subunit
Mice
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Phenotype
Phosphoproteins / genetics
Phosphoproteins / metabolism
Receptors, CXCR4 / genetics
Receptors, CXCR4 / metabolism
Receptors, Interleukin-2 / genetics
Receptors, Interleukin-2 / metabolism
Repressor Proteins / genetics
Repressor Proteins / metabolism
Reproducibility of Results
Spleen / cytology*
Spleen / immunology
Suppressor of Cytokine Signaling 1 Protein
Suppressor of Cytokine Signaling Proteins

Substances

Carrier Proteins
DNA-Binding Proteins
IL2RG protein, human
IRF1 protein, human
IRF7 protein, human
Il2rg protein, mouse
Interferon Regulatory Factor-1
Interferon Regulatory Factor-7
Interleukin Receptor Common gamma Subunit
Irf1 protein, mouse
Irf7 protein, mouse
Phosphoproteins
Receptors, CXCR4
Receptors, Interleukin-2
Repressor Proteins
Socs1 protein, mouse
Suppressor of Cytokine Signaling 1 Protein
Suppressor of Cytokine Signaling Proteins