Abstract
In this report, we describe a short peptide, containing a T helper- and a B-cell epitope, located in the Gag protein of the caprine arthritis encephalitis virus (CAEV). This T-cell epitope is capable of inducing a robust T-cell proliferative response in vaccinated goats with different genetic backgrounds and to provide help for a strong antibody response to the B-cell epitope, indicating that it may function as a universal antigen-carrier for goat vaccines. The primary immune response of goats homozygous for MHC class I and II genes showed an MHC-dependent partitioning in rapid-high and slow-low responses, whereas the memory immune response was strong in both groups, demonstrating that a vaccine based on this immunodominant T helper epitope is capable to overcome genetic differences.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibody Formation / genetics
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Antibody Formation / immunology
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Arthritis-Encephalitis Virus, Caprine / immunology*
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B-Lymphocytes / immunology*
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Cell Proliferation / drug effects
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Enzyme-Linked Immunosorbent Assay
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Gene Products, gag / immunology*
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Goats / immunology*
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Haplotypes
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Hemocyanins / immunology
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Immunization Schedule
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Immunodominant Epitopes / immunology*
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Immunologic Memory / genetics*
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Immunologic Memory / immunology*
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Major Histocompatibility Complex / genetics*
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Major Histocompatibility Complex / immunology*
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Molecular Sequence Data
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Peptides / chemistry
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Peptides / immunology
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Protein Structure, Secondary
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T-Lymphocytes / immunology*
Substances
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Gene Products, gag
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Immunodominant Epitopes
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Peptides
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Hemocyanins
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keyhole-limpet hemocyanin