A new mutation of the tau gene, G303V, in early-onset familial progressive supranuclear palsy

Arch Neurol. 2005 Sep;62(9):1444-50. doi: 10.1001/archneur.62.9.1444.

Abstract

Background: Progressive supranuclear palsy (PSP) is a clinicopathological syndrome related to tau deposits and in linkage disequilibrium with tau polymorphisms. Some rare familial PSP cases have been related to tau gene mutations.

Objective: To present the clinical, pathological, and molecular data of one family with early-onset autosomal dominant PSP.

Design: We performed clinical examinations, quantitative neurological tests, positron emission tomographic scans with fluorodopa F 18 and raclopride C 11, analysis of tau mutations, neuropathological examinations, and protein analyses on brain specimens.

Results: Three family members had PSP confirmed by pathological features in the proband. A novel mutation of tau, G303V, was found in the proband and other family members. tau Isoforms with 4 microtubule-binding repeats were overexpressed in the proband brain.

Conclusions: The G303V mutation of tau is associated with autosomal dominant PSP. Expression of 4 microtubule-binding repeat tau isoforms is increased in the proband.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Blotting, Northern
  • Blotting, Western / methods
  • Brain / metabolism
  • DNA Mutational Analysis / methods
  • Dihydroxyphenylalanine / analogs & derivatives
  • Dihydroxyphenylalanine / metabolism
  • Family Health*
  • Female
  • Genetic Predisposition to Disease
  • Glycine / genetics*
  • Humans
  • Immunohistochemistry / methods
  • Middle Aged
  • Mutation
  • Neurologic Examination
  • Positron-Emission Tomography / methods
  • Protein Isoforms / genetics
  • RNA, Messenger / biosynthesis
  • Raclopride / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Supranuclear Palsy, Progressive / genetics*
  • Supranuclear Palsy, Progressive / pathology
  • Valine / genetics*
  • tau Proteins / genetics*

Substances

  • Protein Isoforms
  • RNA, Messenger
  • tau Proteins
  • fluorodopa F 18
  • Raclopride
  • Dihydroxyphenylalanine
  • Valine
  • Glycine