Blunted inflammatory response to lipopolysaccharide endotracheal instillation due to thermal preconditioning

Minerva Anestesiol. 2005 Oct;71(10):601-7.
[Article in English, Italian]

Abstract

Aim: Heat shock proteins (HSPs), induced by thermal stress, make the cells more resistant to following stress. We aimed to verify if rats heating reduces the production of TNF-alpha in a model of acute respiratory distress syndrome (ARDS).

Methods: We divided 34 Wistar rats in Group A and Group B. The rats of Group A received thermal shock and tracheal instillation of lipopolysaccharide (LPS), the rats of Group B only tracheal instillation of LPS. All the rats have been then subdivided in 6 subgroups sacrificed at different times after instillation of LPS: A30 and B30 at 30 min, A90 and B90 at 90 min and A180 and B180 at 180 min. We dosed the serum concentration of TNF-alpha and the values of every subgroup have been compared with the Student t-test.

Results: The serum concentration of TNF-alpha in the subgroup A90 resulted significantly lower than in the subgroup B90.

Conclusions: The exposure to thermal stress resulting in the production of HSPs reduced the concentration of TNF-alpha in this model of ARDS.

MeSH terms

  • Animals
  • Body Temperature*
  • Disease Models, Animal
  • Hot Temperature*
  • Instillation, Drug
  • Lipopolysaccharides / administration & dosage
  • Lipopolysaccharides / pharmacology*
  • Male
  • Rats
  • Rats, Wistar
  • Respiratory Distress Syndrome / immunology
  • Trachea
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Tumor Necrosis Factor-alpha / drug effects

Substances

  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha