High resolution of multiple forms of red blood cell enzymes using a Toyopearl DEAE 650 S

V Stocchi; L Masat; B Biagiarelli; A Accorsi; G Piccoli; F Palma; L Cucchiarini; M Dachà

doi:10.1080/10826069208018017

High resolution of multiple forms of red blood cell enzymes using a Toyopearl DEAE 650 S

Prep Biochem. 1992 Mar;22(1):11-40. doi: 10.1080/10826069208018017.

Authors

V Stocchi¹, L Masat, B Biagiarelli, A Accorsi, G Piccoli, F Palma, L Cucchiarini, M Dachà

Affiliation

¹ Istituto di Chimica Biologica, Giorgio Fornaini, Università degli Studi, Urbino, Italy.

PMID: 1620685
DOI: 10.1080/10826069208018017

Abstract

We have investigated a new anion exchange chromatographic support (Toyopearl DEAE 650 S) which simultaneously allows easily to remove hemoglobin from hemolysates and to obtain a very high resolution of enzymes present in multiple forms. The results obtained are better than those obtainable using an anion-exchange HPLC column. The data obtained at analytical level suggest a wider use of this new matrix also for preparative purposes without significant changes in the resolution.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anion Exchange Resins
Chromatography, High Pressure Liquid / methods*
Chromatography, Ion Exchange / methods*
Erythrocytes / enzymology*
Ethanolamines
Hexokinase / isolation & purification
Humans
Isoenzymes / isolation & purification*
Polymers
Purine-Nucleoside Phosphorylase / isolation & purification
Rabbits
Reticulocytes / enzymology
Swine

Substances

Anion Exchange Resins
DEAE-Toyopearl 650S
Ethanolamines
Isoenzymes
Polymers
Purine-Nucleoside Phosphorylase
Hexokinase