Characterization of pepsinogen C as a potential biomarker for gastric cancer using a histo-proteomic approach

Christian Melle; Günther Ernst; Bettina Schimmel; Annett Bleul; Roland Kaufmann; Merten Hommann; Konrad K Richter; Wolfgang Daffner; Utz Settmacher; Uwe Claussen; Ferdinand von Eggeling

doi:10.1021/pr050123o

Characterization of pepsinogen C as a potential biomarker for gastric cancer using a histo-proteomic approach

J Proteome Res. 2005 Sep-Oct;4(5):1799-804. doi: 10.1021/pr050123o.

Authors

Christian Melle¹, Günther Ernst, Bettina Schimmel, Annett Bleul, Roland Kaufmann, Merten Hommann, Konrad K Richter, Wolfgang Daffner, Utz Settmacher, Uwe Claussen, Ferdinand von Eggeling

Affiliation

¹ Core Unit Chip Application (CUCA), Institute of Human Genetics and Anthropology, Friedrich Schiller University, 07740 Jena, Germany.

PMID: 16212435
DOI: 10.1021/pr050123o

Abstract

We analyzed 74 cryostat sections of central gastric tumor, tumor margin, and normal gastric epithelium using ProteinChip Arrays and SELDI-TOF MS. One peak was significantly down-regulated in tumor tissue (P = 1.43 x 10(-6)) and identified as pepsinogen C using MS/MS analysis and immunodepletion. This signal was further characterized by immunohistochemistry. This work demonstrates that differentially expressed signals can be identified and assessed using a proteomic approach comprising tissue-microdissection, protein profiling, and immunohistochemistry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / chemistry*
Biomarkers, Tumor
Cell Line, Tumor
Electrophoresis, Gel, Two-Dimensional
Epithelium / metabolism
Gastric Mucosa / metabolism
Gene Expression Regulation, Neoplastic*
Humans
Immunohistochemistry
Lasers
Mass Spectrometry
Pepsinogen C / chemistry*
Peptide Mapping
Protein Array Analysis
Proteome*
Proteomics / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stomach Neoplasms / metabolism*

Substances

Biomarkers
Biomarkers, Tumor
Proteome
Pepsinogen C