Simultaneous display of bacterial and fungal lipases on the cell surface of Bacillus subtilis

Gota Kobayashi; Kaori Fujii; Masakuni Serizawa; Hiroki Yamamoto; Junichi Sekiguchi

doi:10.1016/s1389-1723(02)80047-0

Simultaneous display of bacterial and fungal lipases on the cell surface of Bacillus subtilis

J Biosci Bioeng. 2002;93(1):15-9. doi: 10.1016/s1389-1723(02)80047-0.

Authors

Gota Kobayashi¹, Kaori Fujii, Masakuni Serizawa, Hiroki Yamamoto, Junichi Sekiguchi

Affiliation

¹ Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan.

PMID: 16233158
DOI: 10.1016/s1389-1723(02)80047-0

Abstract

A small cell wall-binding domain (CWB(c)) of the Bacillus subtilis peptidoglycan hydrolase CwlC fused to B. subtilis lipase B was able to be localized on the cell wall of B. subtilis. With the aim of developing an efficient lipid-hydrolyzing bacterium with two different lipases on the cell surface, plasmids possessing genes for LipB-CWB(c) and CWB(b)-CutL (the cell wall-binding domain of CwlB fused to the Aspergillus oryzae lipolytic enzyme CutL) were constructed. B. subtilis harboring these plasmids accumulated considerable amounts of both lipases on the cell surface.