HIV entry inhibitor TAK-779 attenuates atherogenesis in low-density lipoprotein receptor-deficient mice

Arterioscler Thromb Vasc Biol. 2005 Dec;25(12):2642-7. doi: 10.1161/01.ATV.0000192018.90021.c0. Epub 2005 Oct 20.

Abstract

Objective: HIV combination therapy using protease inhibitors is associated with elevated plasma levels of atherogenic lipoproteins and increased risk for atherosclerosis. We investigated whether the HIV entry inhibitor TAK-779 affects lipoprotein levels and atherogenesis in low-density lipoprotein receptor-deficient mice. TAK-779 is an antagonist for the chemokine receptors CCR5 and CXCR3, which are expressed on leukocytes, especially T-helper 1 cells, and these receptors may be involved in recruitment of these cells to atherosclerotic plaques.

Methods and results: TAK-779 treatment of low-density lipoprotein receptor-deficient mice did not elevate the levels of atherogenic lipoproteins, whereas it dramatically reduced atherosclerosis in the aortic root and in the carotid arteries. The number of T cells in the plaque was reduced by 95%, concurrently with a 98% reduction in the relative IFN-gamma area. TAK-779-treated animals showed a decreased percentage of CD4+ and CD8+ T cells in peripheral blood and in mediastinal lymph nodes compared with control-treated animals.

Conclusions: TAK-779 not only suppresses HIV entry via blockade of CCR5 but also attenuates atherosclerotic lesion formation by blocking the influx of T-helper 1 cells into the plaque. TAK-779 treatment may be especially beneficial for young HIV patients as they face lifelong treatment, and this drug impairs atherogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amides / pharmacology*
  • Animals
  • Anti-HIV Agents / pharmacology*
  • Atherosclerosis / drug therapy*
  • Atherosclerosis / genetics
  • Atherosclerosis / immunology
  • CCR5 Receptor Antagonists
  • Chemokine CCL4
  • Chemokine CCL5 / genetics
  • Chemokine CCL5 / metabolism
  • Cholesterol / blood
  • Female
  • Ligands
  • Lymphocyte Count
  • Macrophage Inflammatory Proteins / genetics
  • Macrophage Inflammatory Proteins / metabolism
  • Mice
  • Mice, Mutant Strains
  • Quaternary Ammonium Compounds / pharmacology*
  • RNA, Messenger / analysis
  • Receptors, CCR5 / genetics
  • Receptors, CCR5 / metabolism
  • Receptors, CXCR3
  • Receptors, Chemokine / genetics
  • Receptors, Chemokine / metabolism
  • Receptors, LDL / deficiency
  • Receptors, LDL / genetics*
  • Spleen / drug effects
  • Spleen / immunology
  • Th1 Cells / drug effects*
  • Th1 Cells / physiology

Substances

  • Amides
  • Anti-HIV Agents
  • CCR5 Receptor Antagonists
  • Chemokine CCL4
  • Chemokine CCL5
  • Cxcr3 protein, mouse
  • Ligands
  • Macrophage Inflammatory Proteins
  • Quaternary Ammonium Compounds
  • RNA, Messenger
  • Receptors, CCR5
  • Receptors, CXCR3
  • Receptors, Chemokine
  • Receptors, LDL
  • Cholesterol
  • TAK 779