Surfactin C inhibits the lipopolysaccharide-induced transcription of interleukin-1beta and inducible nitric oxide synthase and nitric oxide production in murine RAW 264.7 cells

Biotechnol Lett. 2005 Oct;27(20):1605-8. doi: 10.1007/s10529-005-2515-1.

Abstract

The anti-inflammatory activity of the surfactin C derived from Bacillus subtilis isolate was investigated in lipopolysaccharide (LPS, 1 microg ml(-1))-treated mouse RAW 264.7 cells. LPS increased mRNA transcription of cyclooxygenase (COX)-2, interleukin (IL)-1beta and inducible nitric oxide synthase (iNOS). However, surfactin C at 50 microg ml(-1 )inhibited the LPS-induced increase in the transcription of IL-1beta and iNOS and nitric oxide (NO) production in a dose-dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacillus subtilis / chemistry*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / pharmacology*
  • Cell Line
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects*
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / genetics
  • Lipopeptides
  • Lipopolysaccharides / pharmacology
  • Mice
  • Nitric Oxide Synthase Type II / biosynthesis*
  • Nitric Oxide Synthase Type II / genetics
  • Peptides, Cyclic / chemistry
  • Peptides, Cyclic / pharmacology*
  • Transcription, Genetic / drug effects

Substances

  • Bacterial Proteins
  • Interleukin-1
  • Lipopeptides
  • Lipopolysaccharides
  • Peptides, Cyclic
  • surfactin peptide
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse