[The expression and the significance of L-FABP and FATP4 in the development of nonalcoholic fatty liver disease in rats]

Zhonghua Gan Zang Bing Za Zhi. 2005 Oct;13(10):776-9.
[Article in Chinese]

Abstract

Objective: To study the effect of liver fatty acid binding protein(L-FABP) and fatty acid transport protein (FATP4) in the development of nonalcoholic fatty liver disease (NAFLD) in rats.

Methods: The expression of L-FABP and FATP4 genes was examined in fatty liver rats by reverse transcription and polymerase chain reaction amplification and Western blot methods.

Results: In the high fat diet group (F), mRNA and protein expression of L-FABP and FATP4 were increased at 2 weeks, and they increased remarkably at 12 weeks (P < 0.05; L-FABP mRNA F=124.9, protein expression F=92.6; FATP4 mRNA F=602.9, protein expression F=108.8).

Conclusion: The high expression of L-FABP and FATP4 at the early stage is an adaptive reaction of the body, With the advanced expression of the L-FABP and FATP4, it can lead to a fatty acid disequilibrium and then result in nonalcoholic fatty liver disease in the rats.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Fatty Acid Transport Proteins / biosynthesis*
  • Fatty Acid Transport Proteins / genetics
  • Fatty Acid-Binding Proteins / biosynthesis*
  • Fatty Acid-Binding Proteins / genetics
  • Fatty Liver / metabolism*
  • Male
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Random Allocation
  • Rats
  • Rats, Wistar

Substances

  • Fatty Acid Transport Proteins
  • Fatty Acid-Binding Proteins
  • RNA, Messenger
  • SLC27A4 protein, human