Abstract
INI1/hSNF5/BAF47 is a core component of the hSWI/ SNF ATP-dependent chromatin remodeling complex, and it has been implicated in regulating gene expression, cell division and tumorigenesis. We investigated whether INI1/hSNF5/BAF47 functions in activation of the colony stimulating factor 1 (CSF1) promoter in HeLa cells. Overexpression of INI1/hSNF5/BAF47 promoted CSF1 transcription, and siRNA targeting INI1/hSNF5/ BAF47 (siINI1) strongly inhibited the activity of the CSF1 promoter. We demonstrated that all conserved domains of INI1/hSNF5/BAF47 are needed for CSF1 transcription. ChIP experiment showed that INI1/ hSNF5/BAF47 is recruited to the region of the CSF1 promoter. Taken together, these results indicate that INI1/hSNF5/BAF47 is involved in activation of the CSF1 promoter.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Culture Techniques
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Chromatin Assembly and Disassembly / genetics
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins / antagonists & inhibitors
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Gene Expression Regulation / physiology*
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HeLa Cells
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Humans
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Macrophage Colony-Stimulating Factor / biosynthesis
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Macrophage Colony-Stimulating Factor / genetics*
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Plasmids / genetics
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Promoter Regions, Genetic
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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RNA, Small Interfering / genetics
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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SMARCB1 Protein
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Transcription Factors / antagonists & inhibitors
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Transcription, Genetic / physiology
Substances
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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RNA, Messenger
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RNA, Small Interfering
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Repressor Proteins
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SMARCB1 Protein
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SMARCB1 protein, human
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Transcription Factors
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Macrophage Colony-Stimulating Factor