A TIM barrel protein without enzymatic activity? Crystal-structure of narbonin at 1.8 A resolution

FEBS Lett. 1992 Jul 13;306(1):80-4. doi: 10.1016/0014-5793(92)80842-5.

Abstract

The major protein component in seeds is storage protein. These have no known enzymatic activity and act to provide amino acids as a source of metabolites in the developing seedling. We report here the first three dimensional crystal structure of a seed storage globulin at high resolution. The molecule of the 2S globulin, narbonin, from Vicia narbonensis L., consists of an eight-stranded parallel alpha/beta barrel structure similar to that observed in triose phosphate isomerase (TIM). Narbonin is the first protein with this topology possessing no known enzymatic activity. Because of the lack of sequence information most of the primary structure was determined directly from the electron density.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Computer Simulation
  • Globulins*
  • Molecular Sequence Data
  • Plant Proteins / chemistry*
  • Plant Proteins / metabolism
  • Plant Proteins, Dietary*
  • Protein Conformation
  • Triose-Phosphate Isomerase / chemistry*
  • Triose-Phosphate Isomerase / metabolism
  • X-Ray Diffraction

Substances

  • Amino Acids
  • Globulins
  • Plant Proteins
  • Plant Proteins, Dietary
  • narbonin
  • Triose-Phosphate Isomerase