Abstract
The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus as an alternative route for glucose metabolism. In this report we demonstrate, by in vitro kinetic studies of D-2-keto-3-deoxygluconate (KDG) kinase and KDG aldolase, that the part-phosphorylative pathway in S. solfataricus is also promiscuous for the metabolism of both glucose and galactose.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Aldehyde-Lyases / analysis
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Aldehyde-Lyases / chemistry
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Aldehyde-Lyases / genetics
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Aldehyde-Lyases / isolation & purification
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Aldehyde-Lyases / metabolism*
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Archaea / metabolism*
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Biotransformation
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Cloning, Molecular
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Escherichia coli / genetics
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Galactose / metabolism
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Genes, Bacterial
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Genome, Bacterial
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Glucose / metabolism
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Kinetics
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Models, Biological
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Molecular Weight
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Peptides / chemistry
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Peptides / metabolism
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Phosphorylation
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Phosphotransferases (Alcohol Group Acceptor) / analysis
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Phosphotransferases (Alcohol Group Acceptor) / chemistry
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Substrate Specificity
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Sulfolobus solfataricus / enzymology*
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Sulfolobus solfataricus / genetics
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Sulfolobus solfataricus / metabolism*
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Temperature
Substances
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Peptides
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Recombinant Proteins
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Adenosine Triphosphate
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Phosphotransferases (Alcohol Group Acceptor)
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2-keto-3-deoxygluconokinase
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Aldehyde-Lyases
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2-keto-3-deoxy-D-glucarate aldolase
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Glucose
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Galactose