Heterologous production of functional forms of Rhizopus oryzae lipase in Escherichia coli

Mirella Di Lorenzo; Aurelio Hidalgo; Michael Haas; Uwe T Bornscheuer

doi:10.1128/AEM.71.12.8974-8977.2005

Heterologous production of functional forms of Rhizopus oryzae lipase in Escherichia coli

Appl Environ Microbiol. 2005 Dec;71(12):8974-7. doi: 10.1128/AEM.71.12.8974-8977.2005.

Authors

Mirella Di Lorenzo¹, Aurelio Hidalgo, Michael Haas, Uwe T Bornscheuer

Affiliation

¹ Department of Technical Chemistry and Biotechnology, Institute of Chemistry and Biochemistry, Greifswald University, Soldmannstr. 16, D-17487 Greifswald, Germany.

Abstract

To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Escherichia coli / enzymology*
Genetic Vectors
Isoenzymes / genetics
Isoenzymes / metabolism
Kinetics
Lipase / genetics
Lipase / metabolism*
Recombinant Proteins / metabolism
Rhizopus / enzymology*
Rhizopus / genetics

Substances

Isoenzymes
Recombinant Proteins
Lipase