Abstract
Genotoxic effects of acrylamide are supposed to result from oxidative biotransformation to glycidamide. After incubation of rat liver slices with acrylamide we detected free glycidamide using a liquid chromatography tandem mass spectrometric method. Glycidamide formation was diminished in the presence of the cytochrome P450 2E1 inhibitor diallyl sulfide (DAS), which is a specific ingredient of garlic. This may be relevant to human health since the suggested carcinogenic risk of dietary acrylamide may be reduced by concomitant intake of garlic.
MeSH terms
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Acrylamide / metabolism*
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Acrylamide / pharmacokinetics
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Allyl Compounds / isolation & purification
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Allyl Compounds / pharmacology*
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Animals
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Biotransformation / drug effects
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Cytochrome P-450 CYP2E1 / metabolism*
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Epoxy Compounds / metabolism*
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Epoxy Compounds / pharmacokinetics
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Garlic / chemistry*
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In Vitro Techniques
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Liver / enzymology
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Liver / metabolism*
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Male
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Rats
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Rats, Wistar
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Sulfides / isolation & purification
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Sulfides / pharmacology*
Substances
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Allyl Compounds
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Epoxy Compounds
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Sulfides
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Acrylamide
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allyl sulfide
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glycidamide
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Cytochrome P-450 CYP2E1